General description
Rabbit Anti-Alpaca IgG2c HRP Alpaca Heavy chain only Antibody is designed to specifically detect Alpaca IgG2c. It is a monoclonal, recombinant, rabbit IgG Fc fused single chain antibody fragment to Alpaca IgG2c coupled to HRP, and Rabbit Anti-Alpaca IgG2c HRP Alpaca Heavy chain only Antibody reacts with Alpaca IgG2c selectively, no cross-reactivity with Alpaca IgG1/2b.
The biological family Camelidae comprises camels (one-humped Camelus dromedarius and two-humped Camelus bactrianus), llama (Lama glama and Lama guanicoe), and vicugna (Vicugna vicugna and Vicugna pacos). Camelidae contain two kinds of IgG in serum: conventional antibodies (IgG1) containing two light chains and two heavy chains (composed of the VH, CH1, hinge, and CH2 and CH3 domains) and two types of homodimeric heavy-chain antibodies (HCAbs), IgG2 and IgG3, which comprise only H chains: each H chain contains a VHH, hinge, and CH2 and CH3 domains. The smallest intact functional antigen-binding fragment of HCAbs is the single-domain VHH, also known as a nanobody(Nb). Alpaca is also called Vicugna pacos. Alpaca IgG contains IgG1a, IgG1b, IgG2b, IgG2c and IgG3.
Immunogen
Recombinant Alpaca single variable domain secondary nano antibody
Biochem/physiol Actions
Alpaca IgG2c
Isotype: Rabbit IgG
Packaging
100 μg in Glass bottle
Physical form
Lyophilized from 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose
Preparation Note
Add 100uL dH2O or 100uL dH2O with 50% Glycerol to a final concentration of 1mg/mL
After reconstitution, store at –20 °C(Avoid freeze / thaw cycles). Protect from light. Stable for 12 months at -20°C
Other Notes
No cross-reactivity with Alpaca IgG1/2b
| biological source | alpaca |
| recombinant | expressed in HEK 293 cells (Human cell line) |
| conjugate | horse radish peroxidase (HRP) |
| antibody form | affinity purified immunoglobulin |
| antibody product type | secondary antibodies |
| clone | recombinant monoclonal |
| form | lyophilized solid |
| mol wt | 100 kDa |
| species reactivity | (alpaca), Alpaca |
| technique(s) | ELISA: 1:4000-1:10000,western blot: 1:4000-1:10000 |
| size | 0.1 mL |
| color | colorless |
| isotype | VHH |
| shipped in | wet ice |
| storage temp. | 2-8°C |
