产品介绍
Product Description
Terminal transferase (TdT) is a template independent polymerase that catalyzes the addition of deoxynucleotides to the 3' hydroxyl terminus of DNA molecules. It catalyzes the repeated addition of deoxyribonucleotides to oligodeoxyribonucleotides and 3' -Oh of single - and double-stranded DNA. TdT requires an oligodeoxynucleotide of at least three bases as a primer. The addition of Co2+ in the reacton makes tailing more efficient.
技术参数
Specifications
| Form | Liquid |
| Storage buffer | 100mM KAc (pH6.8), 2mM 2-mercaptoethanol, 0.01%Triton X-100 and 50% glycerol. |
| Purity | >90% as determined by SDS-PAGE. |
| Applications | Terminal transferase catalyzes the addition of deoxynucleotides to the 3' hydroxyl terminus of DNA molecules. |
| Endotoxin level | Please contact with the lab for this information. |
| Expression system | |
| Protein length | Terminal Transferase (TdT) is expressed in E. coli. |
| Nature | Recombinant |
| Predicted molecular weight | 45 kDa |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt. |
| Reconstitution | |
| Alternative Names | DNA nucleotidylexotransferase, 2.7.7.31, Terminal addition enzyme, Terminal deoxynucleotidyltransferase, TDT, Terminal transferase, DNTT, TDT |
| Species | Bovine |
| Shipping | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note | For research use only. |
